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KMID : 0378019730160080065
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New Medical Journal
1973 Volume.16 No. 8 p.65 ~ p.69
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Electron Microscopic Finding of the Uptake of Protein Antigens by Thoracic Duct Lymphocytes
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劉永奎/Ryu, Young Kyu
林正均/金明國/韓性輸/Lim, Jung Kyun/Kim, Myung Kook/Han, Seong S.
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Abstract
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Throughout this study, Sprague-Dawley (SD) rats weighing approximately 400 to 500 grams, were used. Purified horse ferritin was chosen as the antigen.
Collected lymphocytes were used within three hours after the initial collection began.
They were diluted to a concentration of 5X10" cells Per ml in McCoy´s media at Ph 7.2 and incubated with 10 mg of the purified ferritin for 30 minutes at 37°C.
The cells were then washed 3 times in the same media by centrifuging in a clinical centrifuge. Some of the lymphocytes were centrifuged and fixed in a mixture of 1% paraformaldelhyde and 1% osmium tetroxide in 0.1 M cacodylate buffer at Ph 7.4.
The pellets were fixed for 3 to 4 hours and washed in the same buffer containing 4% sucrose, dehydrated through a graded alcohol and embedded in epoxy resin in a routine manner.
The pellets were sectioned on an LKB ultramicrotome and observed either in a Hitachi HS-8 or Philips 300 electron microscope.
The present studies were undertaken the assess that thoracic duct lymphocytes are capable´ of taking up small protein antigens by pinocytosis using the electron microscope. The results were as follows;
1. Electron microscopic observation of the final cell fraction ciearlye indicated that the cells were relatively free of absorved ferritin molecules as indicated by the absence of ferritin along the cell surface but rather cells indeed ingested ferritin granules by pinocytosis as suggested previously.
2. Thus it could be concluded that thoracic duct lymphocytes are capable of taking up small protein antigens by pinocytosis and that such uptake is immunologically meaningful.
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